Decrease of a peptide in the cat spinal cord after upper cervical transection.

I Kanazawa; D Sutoo

doi:10.1016/0304-3940(81)90335-9

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Decrease of a peptide in the cat spinal cord after upper cervical transection.

I Kanazawa·D Sutoo

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Abstract

The effects of a transection on the peptide contents in the cat cervical spinal cord have been investigated. Seven days following the hemisection at the C1 level, the C5-8 levels of the spinal cord were used for the biochemical analyses. A crude peptide extract was fractionated on a Sephadex G-15 column. The third major peak which contained smaller molecular weight peptides was collected and separated with a peptide map on the cellulose TLC plate. Three ninhydrin-positive and two ninhydrin-negative peptides were found on the TLC plate. When we compared between the operated side and the control side, a ninhydrin-negative peptide, whose acid hydrolysate was composed of glutamic acid and aspartic acid in a ratio of 1:2, was decreased by approximately 30% in the operated side of the spinal cord.

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Animals Cats Chromatography, Thin Layer Peptides Spinal Cord Comparative Study Journal Article Research Support, Non-U.S. Gov't

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I Kanazawa, D Sutoo. 1981-10-23. Decrease of a peptide in the cat spinal cord after upper cervical transection.. https://doi.org/10.1016/0304-3940(81)90335-9

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