PubMed · 650424
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G A Hay. 1978. X-ray imaging.. https://doi.org/10.1088/0022-3735%2F11%2F5%2F029
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The usefulness of the moving intermediate zone concept for improving the performance of the standardized disk agar-diffusion test in measuring susceptibility of the fluorescent group of pseudomonads to gentamicin was studied. For this purpose, a 3-mm moving intermediate zone lying --3 mm and --6 mm below that measured for the quality control microorganism Pseudomonas aeruginosa, ATCC 27853, and a wider moving intermediate zone lying --2 mm and --7 mm below that for the quality control microorganism were investigated. Data from the authors' previous study of the usefulness of using fixed breakpoints and fixed zones for assessing susceptibility of the fluorescent pseudomonads to gentamicin were used for this analysis. The results indicate that both the 3-mm moving intermediate zone and the wider 5-mm moving intermediate zone produced unacceptably high rates of error for testing the susceptibility of the fluorescent group of pseudomonads to gentamicin by the standardized disk agar-diffusion test.
Guard cells of onion irradiated with broad-band blue light display a green intrinsic fluorescence. The fluorescence has been found in eleven species of Allium, but it has not been observed in any other monocot or dicot examined. The fluorescence occurs only in guard cells and is absent in neighbouring epidermal cells. During development it is first apparent in guard mother cells soon after the asymmetric division. Microscopic observation reveals that the fluorescence is associated with the vacuole and examination of vacuoles isolated from guard cell protoplasts suggests that it may be localized on the tonoplast. Microspectrophotometric analysis of single cells reveals an emission peak at around 520 nm. Our results are consistent with the view that this blue light receptor is a flavin or flavoprotein and that it might be related to the blue light-enhanced stomatal opening observed in onion.