Insulin binding on cultured chick muscle cells: decrease in binding associated with cell fusion.

S L Nissen; A H Trenkle; P J Bechtel

doi:10.1055/s-2007-1014774

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Insulin binding on cultured chick muscle cells: decrease in binding associated with cell fusion.

S L Nissen·A H Trenkle·P J Bechtel

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Abstract

Binding of 125I-bovine and chicken insulin to cultured embryonic chick skeletal muscle cells was studied. Bovine and chicken insulin bound cultured cells with high affinities of 2.4 X 10(9)M-1 and 4.8 X 10(9)M-1 and low affinities of 2.4 X 10(7)M-1 and 3.7 X 10(7)M-1, respectively. Maximum insulin binding was achieved after 90 min of incubation at 20 degrees C and the maximum value was maintained for an additional 3 hr. Insulin binding increased in a linear manner with increasing nuclei number over a 5-fold range. Maximum insulin binding per nuclei decreased as cell fusion increased between 24 and 72 hr in culture, primarily due to a decrease in the number of low affinity insulin receptors.

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S L Nissen, A H Trenkle, P J Bechtel. 1984. Insulin binding on cultured chick muscle cells: decrease in binding associated with cell fusion.. https://doi.org/10.1055/s-2007-1014774

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