PubMed · 5527141
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A Chakravarti. 1970. Pulmonary opacity.. https://pubmed.ncbi.nlm.nih.gov/5527141/
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Using serum specimens from patients with alveolar hydatidosis (AH) in Hokkaido, we assessed the usefulness of "Echinococcus Western Blot IgG" (the French immunoblot assay, FIA), which has recently been launched from Ldbio Diagnostics (Lyon, France) as new commercial immunoblot assay kit of immunodiagnosis of Echinococcus infections. Eighty serum specimens were used for the present study: 64 preoperative sera and nine postoperative sera, which were taken from AH patients in Hokkaido, and seven sera from persons who were ELISA (enzyme-linked immunosorbent assay)--positive in mass screening which was conducted for checking on Echinococcus infections in Hokkaido since 1982. When the 64 preoperative sera were examined by the Western blotting method (the Hokkaido method of Western blotting, HWB) which had been carried out at Hokkaido Institute of Public Health between 1987 and 1993, it was found that 53 cases were positive and six cases were quasi-positive, i.e. the rate of the positive cases including quasi-positive cases was 92.2%. From immunostaining patterns, HWB-positive sera could be grouped in two types: the complete type, which showed a pattern of multiple bands containing the 55 and 66 kDa bands, and the incomplete type, which showed patterns of only few bands containing the AH-specific polysaccharide antigen named C antigen. Forty-three of the 53 HWB-positive sera were of the complete type and the residue was of the incomplete type. On the other hand, when the 64 preoperative sera were examined by FIA, 60 sera (93.8%) were judged to be positive and the others as negative sera. On the basis of the interpretation of immunostaining patterns described in the instruction manual, 47 (78.3%) of the 60 positive sera were regarded as pattern P3, five (8.3%) as pattern P4, and eight (13.3%) as pattern P5. All of the complete-type sera were regarded as P3, indicating high antibody titers. Contrarily, most of the incomplete-type or quasi-positive sera resulted in other patterns such as P4 and P5, indicating low antibody titers. Of 5 HWB-negative sera, two were FIA-positive (which showed P3 and P5 patterns respectively), however their immunoreactions were significantly low. Therefore, apart from interpretation of pathological conditions of cases with exceedingly low antibody titers, FIA may be able to give a serologically clear interpretation to HWB-quasi-positive cases, indicating that it is a highly sensitive and useful method for immunodiagnosis of Echinococcus infections.
The authors of this study have examined some experience gained in surgically treating 151 patients with bilateral pulmonary hydatid disease in 1991 to 2001, i.e. over 11 years. The examinees were divided into 7 groups. A hundred and forty patients underwent 205 surgical interventions. With this, organ-preserving operations and resections were made in 198 and 7 cases, respectively. Postoperative complications were observed in 12 cases. There were 3 fatal cases in the postoperative period.