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PubMed · 460180

Postribosomal complexes containing eukaryotic initiation factor eIF-2.

H Amesz·T Haubrich·H O Voorma
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Abstract

Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.

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AnimalsKineticsMacromolecular SubstancesMolecular WeightPeptide Initiation FactorsRabbitsReticulocytesJournal Article

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H Amesz, T Haubrich, H O Voorma. 1979-05-31. Postribosomal complexes containing eukaryotic initiation factor eIF-2.. https://doi.org/10.1007/bf00777498

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