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PubMed · 42594797

Efficacy of the NMIC-150 system in identifying extended-spectrum beta-lactamases in clinical isolates.

Yana Li·Yan Gao·Jingxin Ma·Liyan Ma
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Abstract

Extended-spectrum beta-lactamases (ESBLs) are significant contributors to the growing global crisis of antimicrobial resistance. This study evaluated the performance of the NMIC-150 System for susceptibility testing of third-generation cephalosporins (3GCs) and assessed whether ceftazidime-avibactam and aztreonam-avibactam could identify ESBL-producing carbapenem-resistant Enterobacterales (CREs). A total of 278 non-duplicate clinical isolates (Klebsiella pneumoniae, E. coli, and Proteus mirabilis) were analyzed. Antimicrobial susceptibility was determined using reference broth microdilution (BMD) and the NMIC-150 System. ESBL production was defined as an ≥eight-fold reduction in the minimum inhibitory concentration (MIC) of 3GCs in the presence of clavulanic acid, according to CLSI criteria. Whole-genome sequencing was performed to characterize ESBL and carbapenemase genes among 3GC-resistant isolates. A Random Forest model was used to predict ESBL-producing isolates based on MIC values. The NMIC-150 System demonstrated over 90% categorical and essential agreement with BMD for ceftazidime and ceftriaxone, along with robust predictive performance via Random Forest analysis. These findings suggest that the NMIC-150 System is a reliable platform for 3GC susceptibility testing and that an ≥eight-fold MIC reduction with ceftazidime-avibactam or aztreonam-avibactam may serve as a phenotypic indicator of ESBL production in CRE isolates. In conclusion, the NMIC-150 System shows potential for routine antimicrobial resistance surveillance and may facilitate the rapid identification of ESBL-producing CREs in clinical settings.

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Microbial Sensitivity Testsbeta-LactamasesHumansThird Generation CephalosporinsCeftazidimeAnti-Bacterial AgentsAztreonamAzabicyclo CompoundsDrug CombinationsKlebsiella pneumoniaeProteus mirabilisEscherichia coliWhole Genome SequencingEnterobacteriaceaeEnterobacteriaceae InfectionsBacterial ProteinsAntimicrobial susceptibilityAztreonam-avibactamCeftazidime-avibactamEnterobacteralesExtended-spectrum-beta-lactamases (ESBLs)Journal Article

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Yana Li, Yan Gao, Jingxin Ma, Liyan Ma. 2026-07-26. Efficacy of the NMIC-150 system in identifying extended-spectrum beta-lactamases in clinical isolates.. https://doi.org/10.1016/j.diagmicrobio.2026.117578

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Journal Article resourceAnti-Bacterial Agents subjectBacterial Proteins subjectDrug Combinations subjectEscherichia coli subjectHumans subjectJournal Article subjectKlebsiella pneumoniae subjectMicrobial Sensitivity Tests subjectbeta-Lactamases subject

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