Molecular characterization of 16 MAPK genes in silver carp (Hypophthalmichthys molitrix) and the differences of their mRNA expression between Qiandao Lake and Taihu Lake.

Lianan Wen; Zhaosheng Sun; Yushuai Xie; Jiayong Pan; Long Ren; Guangxi He; Qigen Liu; Qian Gao

doi:10.1016/j.cbd.2026.101967

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Molecular characterization of 16 MAPK genes in silver carp (Hypophthalmichthys molitrix) and the differences of their mRNA expression between Qiandao Lake and Taihu Lake.

Lianan Wen·Zhaosheng Sun·Yushuai Xie·Jiayong Pan·Long Ren·Guangxi He·Qigen Liu·Qian Gao

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Abstract

Mitogen-activated protein kinase (MAPK), a serine-threonine protein kinase, is involved in a variety of stress-induced responses and also plays an important regulatory role in cell metabolism. In the study the open reading frames (ORFs) of 16 MAPK genes in silver carp (Hypophthalmichthys molitrix) were obtained and verified, with the evaluations of their taxonomy, structures, conserved motifs, and evolutionary linkages. And the expression patterns of these genes in the silver carp from Qiandao Lake and Taihu Lake were explored for better understanding the response of MAPK genes to different water environment. MAPK genes of silver carp were divided into three subfamilies, including extracellular signal-regulated kinase (ERK) subfamily, p38 subfamily and C-Jun N-terminal kinase (JNK) subfamily. All these genes possessed similar structures and conserved motifs of MAPK family. Realtime qPCR revealed that the expression patterns of 10 MAPK genes (ScMAPK1, ScMAPK3, ScMAPK4, ScMAPK7, ScMAPK15, ScMAPK8a, ScMAPK8b, ScMAPK9, ScMAPK10 and ScMAPK11) in head kidney, spleen and gill of silver carp in Taihu Lake and Qiandao Lake were different. These findings provide a basis for further research on the function of MAPK in silver carp.

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Animals Carps Lakes RNA, Messenger Mitogen-Activated Protein Kinases Phylogeny Fish Proteins Amino Acid Sequence Expression analysis Hypophthalmichthys molitrix MAPK gene family Molecular characterization Journal Article

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Lianan Wen, Zhaosheng Sun, Yushuai Xie, Jiayong Pan, Long Ren, Guangxi He, Qigen Liu, Qian Gao. 2026-08-06. Molecular characterization of 16 MAPK genes in silver carp (Hypophthalmichthys molitrix) and the differences of their mRNA expression between Qiandao Lake and Taihu Lake.. https://doi.org/10.1016/j.cbd.2026.101967

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Journal Article resource Amino Acid Sequence subject Animals subject Carps subject Expression analysis subject Fish Proteins subject Hypophthalmichthys molitrix subject Journal Article subject Lakes subject MAPK gene family subject Mitogen-Activated Protein Kinases subject Molecular characterization subject

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