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Discovery and characterization of multifunctional bioactive peptides from Alaska Pollock (Gadus chalcogrammus) milt: hybrid in silico, in vitro, and proteomic approaches.

Woo-Jae Choung·Bum Jin Kim·Jung Yeon Kwon
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Abstract

The growing demand for multifunctional bioactive peptides has sparked interest in underutilized marine by-products as sustainable bioresources. This study explored Alaska Pollock (Gadus chalcogrammus) milt protein as a novel source of peptides with anti-inflammatory, anti-hypertensive, and anti-diabetic effects. Protein composition was analyzed via LC-MS, followed by in silico digestion and bioactivity prediction. Molecular docking identified peptides targeting DPP-IV, α-glucosidase, ACE, GLP-1 receptor, COX-2, MuRF1, and the 20S proteasome. Among the candidates, a promising peptide (CLPPH) was synthesized and validated in vitro, demonstrating inhibitory effects on nitric oxide production, DPP-IV, ACE, and α-glucosidase. These results highlight CLPPH's potential as a multifunctional bioactive peptide and support the valorization of Alaska Pollock milt as a sustainable source for functional foods and nutraceutical applications.

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AnimalsProteomicsMolecular Docking SimulationGadiformesPeptidesFish ProteinsBioactive Peptides, DietaryAmino Acid SequenceHumansAnti-Inflammatory AgentsHypoglycemic AgentsMolecular Sequence DataAntihypertensive AgentsAlaska PollockAnti-diabetesAnti-hypertensionAnti-inflammationBioactive peptidesMiltMolecular dockingJournal Article

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Woo-Jae Choung, Bum Jin Kim, Jung Yeon Kwon. 2026-07-26. Discovery and characterization of multifunctional bioactive peptides from Alaska Pollock (Gadus chalcogrammus) milt: hybrid in silico, in vitro, and proteomic approaches.. https://doi.org/10.1016/j.foodchem.2026.150471

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Journal Article resourceAmino Acid Sequence subjectAnimals subjectAnti-Inflammatory Agents subjectFish Proteins subjectHumans subjectJournal Article subject

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