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Carboxyl group number and acidity of organic acids regulate structural reorganization and low glycemic index in cassava pyrodextrins via molecular interactions.

Yifei Dong·Yunlong Cui·Li Li·Hongye Li·Juncai Leng·Wei Zhao
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Abstract

Transforming high-glycemic cassava starch into functional dietary fiber via pyrodextrinization is a promising way to valorize tuber crops, yet the molecular mechanisms catalyzed by organic acids with different carboxyl numbers and acidity remain unclear. This study investigates how carboxyl number and acidity of acetic acid (AA), tartaric acid (TA), and citric acid (CA) affect structural reorganization and low glycemic properties of cassava pyrodextrins. Compared with AA, TA, and CA with stronger acidity and more carboxyl groups promoted more extensive hydrolysis, transglycosylation, repolymerization, and esterification. These changes increased indigestible glycosidic linkages and the branching degree, while reducing molecular weight. Molecular docking confirmed stronger hydrogen-bonding interactions between TA/CA and starch chains. Furthermore, TA- and CA-catalyzed pyrodextrins exhibited superior anti-digestive properties with resistant starch up to 54.26% and an estimated glycemic index as low as 42.46, highlighting the critical role of carboxyl numbers and acidities in modulating the functionality of pyrodextrins.

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ManihotGlycemic IndexMolecular Docking SimulationStarchCitric AcidTartratesAcetic Acid(1)H NMRCassava starchIn vitro digestibilityMolecular docking simulationOrganic acidsPyrodextrinsJournal Article

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Yifei Dong, Yunlong Cui, Li Li, Hongye Li, Juncai Leng, Wei Zhao. 2026-07-13. Carboxyl group number and acidity of organic acids regulate structural reorganization and low glycemic index in cassava pyrodextrins via molecular interactions.. https://doi.org/10.1016/j.foodchem.2026.150396

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