Integrated transcriptomic and metabolomic analyses reveal key regulators associated with lipid metabolic differences between subcutaneous and visceral adipose tissues in sheep.

Siyuan Wang; Yue Pang; Fengjiao Li; Fengshuo Zhang; Qi Wang; Zhongling Chen; Shanshan Zhang; Chengjiao Li; Wuyunga Ma; Menghui Tao; Feng Song; Xingjian Xu; Wenju Zhang

doi:10.1016/j.ygeno.2026.111293

PubMed · 42442620

Integrated transcriptomic and metabolomic analyses reveal key regulators associated with lipid metabolic differences between subcutaneous and visceral adipose tissues in sheep.

Siyuan Wang·Yue Pang·Fengjiao Li·Fengshuo Zhang·Qi Wang·Zhongling Chen·Shanshan Zhang·Chengjiao Li·Wuyunga Ma·Menghui Tao·Feng Song·Xingjian Xu·Wenju Zhang

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Abstract

The location of fat deposition has a significant impact on meat quality and body health, and different adipose tissues exhibit significant differences in lipid metabolism and immune regulation. This study aimed to systematically compare the phenotypic characteristics, transcriptome, and metabolome of subcutaneous adipose tissue (SAT) and two types of visceral adipose tissue (VAT) in sheep, in order to reveal the metabolic differences between SAT and VAT and their potential regulatory mechanisms. The results showed that compared with VAT, SAT had stronger triglyceride deposition ability and obvious cellular hypertrophy. Through integrative analysis, 15 key lipid metabolism genes and 12 differential metabolites were identified. Among them, ACACA, FASN, ELOVL6, SCD, as well as metabolites palmitic acid and glycerol-3-phosphate, may play a central role in SAT lipid synthesis and storage; whereas IGFBP2, ADRB3, LTA4H, and metabolites arachidonic acid and leukotriene B4 may be involved in the lipolysis regulation and inflammatory response of VAT. These findings may provide deeper insights into the regulatory mechanisms of fat deposition in sheep.

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Animals Intra-Abdominal Fat Lipid Metabolism Sheep Subcutaneous Fat Metabolome Transcriptome Lipid metabolomics Multi-omics Subcutaneous adipose tissue Visceral adipose tissue Journal Article

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Siyuan Wang, Yue Pang, Fengjiao Li, Fengshuo Zhang, Qi Wang, Zhongling Chen, Shanshan Zhang, Chengjiao Li, Wuyunga Ma, Menghui Tao, Feng Song, Xingjian Xu, Wenju Zhang. 2026-07-13. Integrated transcriptomic and metabolomic analyses reveal key regulators associated with lipid metabolic differences between subcutaneous and visceral adipose tissues in sheep.. https://doi.org/10.1016/j.ygeno.2026.111293

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