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Cross-kingdom dynamics of the subgingival bacteriome and mycobiome: A pilot study on the effects of a novel HA-H₂O₂-Glycine formulation to treat periodontitis.

Chiara Gini·Marco Farronato·Arianna Manunza·Giulia Ravasi·Pamela Abbruscato·Erna Cecilia Lorenzini·Alessandra Stella
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Abstract

OBJECTIVES: Traditional periodontal therapy primarily focuses on bacterial biofilm control; however, recent evidence also suggests a critical role for the oral mycobiome. This study evaluated the clinical and ecological impact of a novel mouthwash formulation containing hyaluronic acid (HA), hydrogen peroxide (H2O2), and glycine on periodontal patients METHODS: This prospective, randomized split-mouth trial included 13 adult participants with periodontitis treated with HA-H2O2-glycine formula (BMG0703A) used twice a day for seven days. Subgingival plaque samples were collected from periodontal pocket and healthy control sites at baseline (T0) and one-week post-treatment (T1). Microbial and fungal communities were characterized using Next-Generation Sequencing (NGS) of the 16S rRNA and ITS2 regions. Linear Mixed Models (LMM) and Spearman correlation were used to assess taxonomic shifts and cross-kingdom relationships. RESULTS: Sequencing revealed a promising ecological shift: the bacteriome shifted from anaerobic dominance (Olsenella, Peptostreptococcus) toward a health-associated aerobic profile, with Rothia near-doubling (11.91% to 22.68%). The mycobiome underwent a "normalization" effect: Candida abundance decreased significantly (22.8% to 9.1%), while fungal Shannon diversity in pockets returned to healthy-site levels. Inter-kingdom analysis identified antagonistic relationships between expanding commensal bacteria and opportunistic fungi, suggesting that the intervention may help re-establish a protective bacterial niche. CONCLUSIONS: The HA-H2O2-glycine formulation seems to facilitate a rapid, cross-kingdom modulation of the subgingival niche. By reducing anaerobic pathogens and normalizing the mycobiome it appear to induce short-term changes, suggesting potential as adjunctive strategy in periodontal management. CLINICAL SIGNIFICANCE: The present work underlines the possible cross-Kingdom effects of a novel compound.

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HumansPilot ProjectsGlycineHydrogen PeroxideMouthwashesDental PlaqueProspective StudiesHyaluronic AcidAdultMycobiomeMaleFemalePeriodontal PocketPeriodontitisGingivaBacteriaRNA, Ribosomal, 16SMicrobiotaFungiGlycine and hydrogen peroxide mouthwashHyaluronic acidMouthwash therapyOral microbiomeOral mycobiomeJournal ArticleRandomized Controlled Trial

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Chiara Gini, Marco Farronato, Arianna Manunza, Giulia Ravasi, Pamela Abbruscato, Erna Cecilia Lorenzini, Alessandra Stella. 2026-07-11. Cross-kingdom dynamics of the subgingival bacteriome and mycobiome: A pilot study on the effects of a novel HA-H₂O₂-Glycine formulation to treat periodontitis.. https://doi.org/10.1016/j.jdent.2026.106905

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Journal Article; Randomized Controlled Trial resourceAdult subjectBacteria subjectFemale subjectFungi subjectGlycine subjectHumans subjectJournal Article subjectMale subjectMicrobiota subjectMouthwashes subjectRandomized controlled trials study

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