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High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).

Ian T Lamborn·Hiroshi Ichise·Emily Speranza·Leanne Arakkal·Vivien I Maltez·Ziv Yaniv·Andrea J Radtke·Ronald N Germain
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Abstract

Iterative bleaching extends multiplexity (IBEX) is an easy-to-use, highly multiplex immunofluorescent tissue imaging method that employs widely available microscopy platforms, commercial reagents, and open-source software. In this article, we describe how to implement this method in a laboratory that has minimal experience with immunohistochemistry.

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SoftwareFluorescent Antibody TechniqueHumansImage Processing, Computer-AssistedMicroscopy, FluorescenceAnimalsImmunohistochemistryProteomicsIBEXImmunofluorescenceMicroscopyMultiplexOptical imagingSpatial proteomicsJournal Article

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Ian T Lamborn, Hiroshi Ichise, Emily Speranza, Leanne Arakkal, Vivien I Maltez, Ziv Yaniv, Andrea J Radtke, Ronald N Germain. 2026. High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).. https://doi.org/10.1007/978-1-0716-5027-1_1

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