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Network pharmacological and experimental validation of the mechanism of Chaihu Guizhi Ganjiang decoction regulating T helper cell 17/regulatory T cell balance to improve autoimmune hepatitis.

Liang Zihao·Gao Jie·Song Jinyun·Zheng Qin·Zhao Hongyu
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Abstract

OBJECTIVE: To elucidate the therapeutic efficacy and mechanism of action of Chaihu Guizhi Ganjiang decoction (, CGGD) in autoimmune hepatitis. METHODS: CGGD components and potential target genes were extracted from previously published databases. The autoimmune hepatitis (AIH)-related regulatory genes were obtained from the DisGeNET database. Intersections were taken, and enrichment analyses were performed on the extracted data. Concanavalin A (ConA)-induced AIH model mice were treated with CGGD via gavage. The results of network pharmacological analysis were experimentally validated. RESULTS: Network pharmacology revealed 228 genes at the intersection of AIH and CGGD. Kyoto Encyclopedia of Genes and Genomes analysis revealed that CGGD primarily regulates the phosphoinositide 3-kinase (PI3K)/ protein kinase B (AKT) signaling pathway and cellular metabolism in AIH. Gene Ontology enrichment analysis revealed that CGGD modulates inflammation through transcription factor-mediated signaling pathways. As predicted, CGGD attenuated ConA-induced AIH in a dose-dependent manner by activating the PI3K/AKT signaling pathway. Histopathological assessment confirmed the protective effects of CGGD against ConA-induced AIH. Further investigation revealed that CGGD regulated the T helper cell 17 (Th17)/regulatory T cell (Treg) balance by modulating the PI3K/Akt/ nuclear factor kappa-B (NF-κB) pathway. CONCLUSIONS: This study demonstrated the therapeutic effect of CGGD on AIH through a combination of network pharmacological prediction and experimental validation. Its mechanism of action involves PI3K/Akt/ NF-κB-mediated regulation of Th17/Treg cells.

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AnimalsDrugs, Chinese HerbalMiceHepatitis, AutoimmuneT-Lymphocytes, RegulatoryTh17 CellsHumansSignal TransductionNetwork PharmacologyPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktMaleChaihu Guizhi Ganjiang decoctionNF-kappa BT helper cell 17autoimmune hepatitisnetwork pharmacologyphosphoinositide 3-kinaseprotein kinase Bregulatory T cellJournal ArticleResearch Support, Non-U.S. Gov't

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Liang Zihao, Gao Jie, Song Jinyun, Zheng Qin, Zhao Hongyu. 2026. Network pharmacological and experimental validation of the mechanism of Chaihu Guizhi Ganjiang decoction regulating T helper cell 17/regulatory T cell balance to improve autoimmune hepatitis.. https://doi.org/10.19852/j.cnki.jtcm.2026.01.010

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