iTRAQ-based quantitative proteomics reveals reduced expression of KRT19, KRT7, and PSTDG in cutaneous specimens after kidney transplantation.

Ichiro Tsuboi; Yosuke Mitsui; Kasumi Yoshinaga; Tomoaki Yamanoi; Takanori Sekito; Yuki Maruyama; Takuya Sadahira; Shingo Nishimura; Kensuke Bekku; Motoo Araki

doi:10.1038/s41598-025-18391-2

PubMed · 41006769

iTRAQ-based quantitative proteomics reveals reduced expression of KRT19, KRT7, and PSTDG in cutaneous specimens after kidney transplantation.

Ichiro Tsuboi·Yosuke Mitsui·Kasumi Yoshinaga·Tomoaki Yamanoi·Takanori Sekito·Yuki Maruyama·Takuya Sadahira·Shingo Nishimura·Kensuke Bekku·Motoo Araki

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Abstract

Clinical improvement in pigmentation is frequently observed after kidney transplantation. However, the underlying molecular and histological mechanisms remain unclear. We conducted a study to quantify the skin color change using a handheld reflected light colorimeter and to investigate protein expression changes in the skin before and after kidney transplantation. Paired skin biopsies were obtained from three patients who underwent kidney transplantation before and one month after transplantation. Protein expression was analyzed using iTRAQ-based quantitative proteomics. Differentially expressed proteins were identified and visualized using hierarchical clustering and volcano plots. Histopathological evaluation included hematoxylin and eosin (H&E), Masson's trichrome, and immunohistochemical (IHC) staining for keratin (KRT) 7, KRT19, and MelanA. Skin pigmentation of the arms, ankles, and abdomen had significant L-value improvement after kidney transplantation. Proteomic profiling identified 2148 proteins, with six proteins showing significant differential expression after transplantation. Among them, KRT7, KRT19, and prostaglandin D2 synthase (PTGDS) were significantly downregulated, potentially reflecting reduced epithelial stress and systemic inflammation. H&E and Masson's trichrome staining revealed a post-transplantation reduction in dermal pigmentation and collagen content. IHC showed decreased KRT7, KRT19, and MelanA expression after transplantation. Our results suggest that targeting KRT or prostaglandin pathways may offer new treatments for ESRD-related skin symptoms.

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Humans Kidney Transplantation Proteomics Keratin-19 Skin Keratin-7 Male Middle Aged Female Skin Pigmentation Adult Cutaneous manifestations Dialysis Keratin Pigmentation Prostaglandin D2 synthase Renal transplantation Skin color Journal Article

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Ichiro Tsuboi, Yosuke Mitsui, Kasumi Yoshinaga, Tomoaki Yamanoi, Takanori Sekito, Yuki Maruyama, Takuya Sadahira, Shingo Nishimura, Kensuke Bekku, Motoo Araki. 2025-09-26. iTRAQ-based quantitative proteomics reveals reduced expression of KRT19, KRT7, and PSTDG in cutaneous specimens after kidney transplantation.. https://doi.org/10.1038/s41598-025-18391-2

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