A reversible decrease in electrical coupling of cultured mouse glial cells induced by superfusion from a micropipette.

H Kettenmann

doi:10.1016/0304-3940(85)90219-8

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A reversible decrease in electrical coupling of cultured mouse glial cells induced by superfusion from a micropipette.

H Kettenmann

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Abstract

Cultured mouse oligodendrocytes were superfused by pressure application to the rear-end of a 3-20 microns micropipette filled with normal bathing fluid. Input resistance was determined during superfusion with two separate electrodes. The input resistance increased reversibly by 90% in 26 cells tested and was unaffected in 39 cells. When pairs of oligodendrocytes were electrically coupled, coupling decreased in a reversible manner during superfusion. Therefore, the flow from a micropipette can uncouple cells, and studies involving application of substances by pressure ejection from microelectrodes must be evaluated with care.

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Animals Cells, Cultured Electric Conductivity Embryo, Mammalian Membrane Potentials Mice Microelectrodes Neuroglia Neuropharmacology Oligodendroglia Journal Article Research Support, Non-U.S. Gov't

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H Kettenmann. 1985-08-16. A reversible decrease in electrical coupling of cultured mouse glial cells induced by superfusion from a micropipette.. https://doi.org/10.1016/0304-3940(85)90219-8

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