PubMed · 40403729
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.
Abstract
The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution of each cell type to the processes guided by the cortex is essential for understanding its function in health and disease. We use transcriptomic and epigenomic cortical cell-type taxonomies from mouse and human to define marker genes and putative enhancers and create a large toolkit of transgenic lines and enhancer adeno-associated viruses (AAVs) for selective targeting of cortical cell populations. We report creation and evaluation of fifteen transgenic driver lines, two reporter lines, and >1,000 different enhancer AAV vectors covering most subclasses of cortical cells. The tools reported here have been made publicly available, and along with the scaled process of tool creation, evaluation, and modification, they will enable diverse experimental strategies toward understanding mammalian cortex and brain function.
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Yoav Ben-Simon, Marcus Hooper, Sujatha Narayan, Tanya L Daigle, Deepanjali Dwivedi, Sharon W Way, Aaron Oster, David A Stafford, John K Mich, Michael J Taormina, Refugio A Martinez, Ximena Opitz-Araya, Jada R Roth, Jason R Alexander, Shona Allen, Adam Amster, Joel Arbuckle, Angela Ayala, Pamela M Baker, Trygve E Bakken, Tyler Barcelli, Stuard Barta, Jacqueline Bendrick, Darren Bertagnolli, Cameron Bielstein, Prajal Bishwakarma, Jessica Bowlus, Gabriella Boyer, Krissy Brouner, Brittny Casian, Tamara Casper, Anish B Chakka, Rushil Chakrabarty, Rebecca K Chance, Sakshi Chavan, Michael Clark, Kaity Colbert, Forrest Collman, Scott Daniel, Maxwell Departee, Peter DiValentin, Nicholas Donadio, Nadezhda Dotson, Tom Egdorf, Tim Fliss, Mariano Gabitto, Jazmin Garcia, Amanda Gary, Molly Gasperini, Jessica Gloe, Jeff Goldy, Bryan B Gore, Lucas Graybuck, Noah Greisman, Francoise Haeseleer, Carliana Halterman, Zeb Haradon, Samantha D Hastings, Olivia Helback, Windy Ho, Dirk Hockemeyer, Cindy Huang, Sydney Huff, Avery Hunker, Nelson Johansen, Danielle Jones, Zoe Juneau, Brian Kalmbach, Madhav Kannan, Shannon Khem, Emily Kussick, Rana Kutsal, Rachael Larsen, Changkyu Lee, Angus Y Lee, Madison Leibly, Garreck H Lenz, Su Li, Elizabeth Liang, Nicholas Lusk, Zachary Madigan, Jessica Malloy, Jocelin Malone, Rachel McCue, Jose Melchor, Tyler Mollenkopf, Skyler Moosman, Elyse Morin, Dakota Newman, Lydia Ng, Kiet Ngo, Victoria Omstead, Sven Otto, Alana Oyama, Nick Pena, Trangthanh Pham, Elliot Phillips, Christina A Pom, Lydia Potekhina, Shea Ransford. 2025-05-21. A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.. https://doi.org/10.1016/j.cell.2025.05.002
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