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Proteomic serum profiles before and after lipoprotein apheresis in patients with peripheral artery disease with ulceration.

Kohei Ishiga·Tatsuki Uehara·Hiromichi Wakui·Kengo Azushima·Eiko Ueda·Daisuke Kanai·Mari Sotozawa·Ryu Kobayashi·Sho Kinguchi·Tomohiko Kanaoka·Tatsuya Haze·Yoshiyuki Toya·Kouichi Tamura
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Abstract

INTRODUCTION: The efficacy of lipoprotein apheresis (LA) in peripheral arterial disease (PAD) has been primarily attributed to its anti-atherosclerotic effects through the adsorption of lipoproteins. However, the other potential effects of LA remain unknown. We evaluated changes in serum profiles before and after LA using a comprehensive analysis to explore the underlying mechanism. METHODS: Ten patients with leg ulcers were included from the LETS-PAD study, in which patients with lipoprotein-controlled PAD underwent LA. Serum samples collected at baseline and 1 month after LA were analyzed for proteomic changes. RESULTS: Six patients exhibited ulcer epithelialization and skin perfusion pressure improvement. Proteomic analysis identified 2033 proteins. Fifty-five proteins showed significant differences. B-cell lymphoma protein-2 associated X (BAX) and C-X-C motif chemokine 10 (CXCL10) were downregulated. CONCLUSION: Serum BAX and CXCL10 levels significantly decreased after LA, which may be involved in the ulcer epithelialization mechanism of LA, which potentially acts through angiogenesis promotion.

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HumansPeripheral Arterial DiseaseBlood Component RemovalMaleFemaleProteomicsAgedLipoproteinsLeg UlcerMiddle Agedbcl-2-Associated X ProteinAged, 80 and overdextran sulfatelipoprotein apheresisperipheral artery diseaseproteome analysisJournal Article

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Kohei Ishiga, Tatsuki Uehara, Hiromichi Wakui, Kengo Azushima, Eiko Ueda, Daisuke Kanai, Mari Sotozawa, Ryu Kobayashi, Sho Kinguchi, Tomohiko Kanaoka, Tatsuya Haze, Yoshiyuki Toya, Kouichi Tamura. 2024-12-30. Proteomic serum profiles before and after lipoprotein apheresis in patients with peripheral artery disease with ulceration.. https://doi.org/10.1111/1744-9987.14247

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