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PubMed · 3812985

Sequential volume changes on a small sample by density.

D W Kupke
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Abstract

A procedure is described for generating a sequence of volume changes by density on the same sample of a solution. The entire volume-change profile over the reactant concentration range of interest can be accomplished with 0.1-0.2 mumol of a protein in 100 microliters of sample. The calcium-binding proteins, calmodulin and skeletal troponin-C, were employed to determine the volume increases attending the sequential addition of calcium ions. Both volume-change profiles exhibited nonlinear increases over the first four equivalents of calcium ion added.

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AnimalsCalciumCalcium-Binding ProteinsCalmodulinCattleMicrochemistryRabbitsSolutionsThermodynamicsTroponinTroponin CJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D W Kupke. 1986-11-01. Sequential volume changes on a small sample by density.. https://doi.org/10.1016/0003-2697(86)90576-2

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