PubMed · 3757234
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D Lloyd, J Marples. 1986. Serum fructosamine and thyroid function.. https://pubmed.ncbi.nlm.nih.gov/3757234/
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The changes of fructosamine (FA) in low density lipoprotein (LDL) incubated in different durations and concentrations of glucose, electrophoretic mobility of different degrees of glycosylated modification of LDL, and the effects on platelet aggregation by glycosylated LDL were observed. The results revealed that FA content increased in LDL incubated with glucose and induced glycosylated modification of LDL, and FA content in LDL correlated to the duration of LDL incubated with glucose and also correlated to different concentrations of glucose. There was a positive correlation between electrophoretic mobility and FA content in LDL. The higher of FA content in LDL the higher of platelet aggregation. This study indicated that FA content in LDL reflected the degree of glycosylated modification of LDL, in which the physical, chemical and biological properties were changed.
The original fructosamine assay, based on reduction of NBT, has been modified and marketed as a kit. The development includes addition of detergents, uricase, change of the buffer and NBT concentrations, and use of a new calibrator. We report the effects of these changes on the measured values of fructosamine in lipemic and uricemic sera. A secondary calibrator produced from pooled serum and stabilized by removal of glucose was used to calibrate the original fructosamine assay and enhance transferability of results. There was no correlation between the difference between the results obtained by the two procedures and the concentration of urate or triglycerides. The development of colour was faster with the original method. It is suggested that measurements of fructosamine by either method are practically equivalent. However, the use of polylysine as a calibrator is advantageous. It is suggested that the generic term S-Glycated proteins is used for the measurand rather than S-Fructosamine which in effect has become a commercial term.
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