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Biomedical subjects

J Marples

Publications and source records attributed to J Marples.

11 recordsLinked to original sources
PubMed1988

Serum fructosamine does reflect levels of glycated serum albumin in insulin-dependent diabetics.

Fructosamine was measured in the serum of 62 patients with insulin-dependent diabetes (IDD) and 32 non-diabetics and the results compared with glycated albumin levels (GSA) measured using the affinity medium Cibarcron blue F3GA. Good correlations were found both for the IDD patients (r = 0.93) and the combined group of IDD plus non-diabetics (r = 0.95). We conclude, that fructosamine measurements accurately reflect GSA concentrations, and, therefore, provide a practical method for assessing intermediate term glycaemia in IDD.

Adolescent↗
PubMed1985

Comparison of serum fructosamine with glycosylated serum protein (determined by affinity chromatography) for the assessment of diabetic control.

Glycosylated total protein (GTP) and glycosylated albumin (GALb) were measured in serum using aminophenylboronic acid affinity chromatography and the results were compared with those found using the fructosamine assay. The percentage GTP and GALb found by affinity chromatography correlated well with fructosamine values in the sera of a group of non-diabetic and diabetic patients (fructosamine vs GTP, r = 0.91, p less than 0.001; fructosamine vs GALb, r = 0.91, p less than 0.001). Results of each method gave similar correlations when compared with the degree of diabetic control assessed by glycosylated haemoglobin (GHb) and fasting plasma glucose (FPG) (fructosamine vs FPG, r = 0.74, p less than 0.001; GTP vs FPG, r = 0.75, p less than 0.001; GALb vs FPG, r = 0.79, p less than 0.001; fructosamine vs GHb, r = 0.79, p less than 0.001; GTP vs GHb, r = 0.81, p less than 0.001; GALb vs GHb, r = 0.84, p less than 0.001). Both methods could equally discriminate between groups of non-diabetics and diabetic patients (p less than 0.001) and showed similar temporal changes after starting insulin therapy.

Adolescent↗
PubMed1984

Simple colorimetry of glycated serum protein in a centrifugal analyzer.

A recently described (Clin Chim Acta 127: 87-95, 1982) colorimetric assay for glycated proteins in serum exploits their ketoamine activity to reduce 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis [2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] (nitroblue tetrazolium) in alkaline solution; the authors termed this the "fructosamine assay." The method is simple, requiring only the addition of one reagent and measurement of the absorbance change during 5 min; results are expressed relative to a synthetic standard. We have adapted this for use in a centrifugal analyzer and report its performance, both analytically and as an index of hyperglycemia. Precision is good (between-batch CV 2.1%), the reagent is stable and inexpensive, and the procedure is rapid (75 samples per hour). Albumin influences the measurement, but for concentrations greater than or equal to 35 g/L this was not a serious problem. Normal and diabetic populations can be clearly discriminated (p less than 0.001). The test detected 25 (84%) of the 30 untreated diabetics studied and gave four false positives (8%). The results correlate well with those for glucose in plasma of fasting subjects (r = 0.87) and for hemoglobin A1 (r = 0.80).

Adult↗