Search PubMed⌕ Search

PubMed · 3529928

Microcomputer-assisted interpretative reporting of sequential cardiac profile data.

Abstract

The authors have developed a microcomputer-based system for interpretative reporting of sequential cardiac profile data, which consists of creatine kinase and lactate dehydrogenase isoenzyme levels. Patient demographic data and test results (total creatine kinase [CK], MB isoenzyme of CK [CK-MB], lactate dehydrogenase isoenzyme 1 [LD-1], and lactate dehydrogenase isoenzyme 2 [LD-2]) are entered manually through the keyboard. Percent MB and LD ratio are calculated. The test results are compared with normal range values, and an interpretative report is generated, including all pertinent demographic information and graphic display of up to 36 previous CK and LD isoenzyme determinations. Interpretative statements are printed beneath the graphic display after analysis of previous test results. The combination of graphic data display and interpretations based on prior data provides useful and accurate information to the cardiologist. Significant discrepancies between computer-assisted and pathologist interpretation were not encountered.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

T S Talamo, F J Losos, D W Mercer. 1986. Microcomputer-assisted interpretative reporting of sequential cardiac profile data.. https://doi.org/10.1093/ajcp%2F86.3.336

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

High-throughput assays for DNA gyrase and other topoisomerases.

We have developed high-throughput microtitre plate-based assays for DNA gyrase and other DNA topoisomerases. These assays exploit the fact that negatively supercoiled plasmids form intermolecular triplexes more efficiently than when they are relaxed. Two assays are presented, one using capture of a plasmid containing a single triplex-forming sequence by an oligonucleotide tethered to the surface of a microtitre plate and subsequent detection by staining with a DNA-specific fluorescent dye. The other uses capture of a plasmid containing two triplex-forming sequences by an oligonucleotide tethered to the surface of a microtitre plate and subsequent detection by a second oligonucleotide that is radiolabelled. The assays are shown to be appropriate for assaying DNA supercoiling by Escherichia coli DNA gyrase and DNA relaxation by eukaryotic topoisomerases I and II, and E.coli topoisomerase IV. The assays are readily adaptable to other enzymes that change DNA supercoiling (e.g. restriction enzymes) and are suitable for use in a high-throughput format.

Clinical Enzyme Tests↗

Prenatal diagnosis for severe methylenetetrahydrofolate reductase deficiency by linkage analysis and enzymatic assay.

Severe methylenetetrahydrofolate reductase (MTHFR) deficiency is characterized by varying degrees of developmental delay, motor and gait abnormalities, seizures, and thrombosis. Biochemical abnormalities include homocystinuria and hyperhomocysteinemia. Clinical severity correlates with MTHFR activity in cultured fibroblasts; activity can also be assayed in cultured amniocytes and chorionic villus cells (CVC). Forty-four private mutations have been identified, limiting the use of direct mutation detection for prenatal diagnosis. However, intragenic polymorphisms have been identified, making prenatal diagnosis by linkage analysis a possible option, even without knowledge of deleterious mutations. Prenatal diagnosis for severe MTHFR deficiency has been available by biochemical methodologies, but molecular genetic approaches have not yet been reported. We performed prenatal diagnosis for severe MTHFR deficiency in 11 at-risk pregnancies in seven families. A combined approach of linkage analysis and enzymatic assays was used in six pregnancies; linkage analysis alone was performed in one pregnancy. Linkage analysis for the 677C > T or 1298A > C polymorphisms predicted that all seven fetuses were unaffected. For six of these seven fetuses, enzymatic activities were also measured and demonstrated concordant results. Of the 10 pregnancies in which enzymatic assays were performed, activities in cultured amniocytes predicted six unaffected fetuses (1.4-7.1 nmol CHO/mg prot/h (U)) and one affected fetus (0.24 U [control 3.1-9.6 U]). Three pregnancies assessed via CVCs demonstrated two unaffected fetuses (3.6 and 7.7 U) and 1 affected fetus (0 U [control 4.5-7.8 U]). These values were compared to those of the probands (range = 0.02-0.7 U (control 2.4-11.7 U)) in cultured fibroblasts. Our findings suggest that linkage analysis for severe MTHFR deficiency can be a practical approach for prenatal diagnosis.

Clinical Enzyme Tests↗