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PubMed · 3516650

Microbiologic studies.

P Pancoast·J B McCabe
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Abstract

Although useful to emergency physicians in determining and following up treatment, the microbiology department can be a source of frustration and erroneous results due to improper or inconsistent methodology, ordering, and interpretation. This article will review the principles of microbiologic specimen collection, handling, and processing as well as noting specific techniques for selected clinical settings.

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Bacteriological TechniquesDiagnostic Tests, RoutineDiarrheaEmergenciesFecesFemaleHumansMalePharyngitisRespiratory Tract InfectionsSepsisSkin DiseasesSputumUrinary Tract InfectionsVaginitisJournal ArticleReview

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P Pancoast, J B McCabe. 1986. Microbiologic studies.. https://pubmed.ncbi.nlm.nih.gov/3516650/

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Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

Bacteriological Techniques↗