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PubMed · 2803732

Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts.

A Teti·M Grano·S L Teitelbaum·K A Hruska·S Colucci·A Zambonin Zallone
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Abstract

In this study the effects of changes in intracellular pH on cytosolic Ca2+ were examined in single isolated osteoclasts. Alkalinization, performed by incubation in HCO3 containing buffer, induced increases in [Ca2+]. Conversely acidification, obtained by incubation in Na-butyrate-containing buffer induced a rapid and sustained decrease of [Ca2+]. The decrease of [Ca2+], during acidification with Na-butyrate was inhibited by VO4, an inhibitor of the Ca2+-ATPase. All these pieces of information indicate that changes in pH1 can modulate osteoclast activity together with modifications of [Ca2+], probably acting as intracellular signals.

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AnimalsBirdsCalciumCytosolHydrogen-Ion ConcentrationIn Vitro TechniquesOsteoclastsJournal Article

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A Teti, M Grano, S L Teitelbaum, K A Hruska, S Colucci, A Zambonin Zallone. 1989. Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts.. https://pubmed.ncbi.nlm.nih.gov/2803732/

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