Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 1901712

Improved method for coliform verification.

J D Diehl
View original source ↗Export metadata ↓

Abstract

Modification of a method for coliform verification presented in Standard Methods for the Examination of Water and Wastewater is described. Modification of the method, which is based on beta-galactosidase production, involves incorporation of a lactose operon inducer in medium upon which presumptive coliform isolates are cultured prior to beta-galactosidase assay.

Explore related subjects

Bacteriological TechniquesEnterobacteriaceaeEvaluation Studies as TopicIsopropyl ThiogalactosideLac OperonWater MicrobiologyWater Supplybeta-GalactosidaseJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

J D Diehl. 1991. Improved method for coliform verification.. https://doi.org/10.1128/aem.57.2.604-605.1991

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

Luminometric measurement of population activity of genetically modified Pseudomonas fluorescens in the soil.

Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

Bacteriological Techniques↗