[Circulating immune complexes in multiple sclerosis].

J Losy; M Wender

PubMed · 1839806

[Circulating immune complexes in multiple sclerosis].

Abstract

Circulating immunocomplexes were detected in sera from MS patients using solid-phase ELISA anti-C3 method. Estimations have revealed positive results in 71% of 21 MS cases. They were positive in 73% of MS cases with relapse-remitting course and in 66% of MS cases with chronic-progressive course compared with 5% among controls. The concentration of immunocomplexes in serum was 62 +/- 4 micrograms/ml in patients with chronic-progressive course and was significantly higher than the concentration of immunocomplexes in patients with relapse-remitting course of the disease--40.1 +/- 5.1 micrograms/ml.

Explore related subjects

Antigen-Antibody Complex Chronic Disease Enzyme-Linked Immunosorbent Assay Humans Immune Complex Diseases In Vitro Techniques Multiple Sclerosis English Abstract Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

J Losy, M Wender. [Circulating immune complexes in multiple sclerosis].. https://pubmed.ncbi.nlm.nih.gov/1839806/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2000

Detection of disease related immune complexes in the serum of leprosy patients. A novel single step method.

Mycobacterium leprae antigen and antibody complexes could be detected in the serum of leprosy patients using monoclonal antibody ML34 and anti-BCG antibodies by enzyme-linked immunosorbent assay. This simplified system detects disease related complexes without the need for isolating and purifying them from the serum. Immune complexes captured using monoclonal antibody ML34 revealed positivity in seven out of eight neuritic, two out of nine tuberculoid (TT), five out of ten borderline tuberculoid (BT), four out of ten borderline lepromatous (BL) and four out of ten lepromatous (LL), leprosy cases. One of the controls also showed immune complex of an IgM type. Anti-BCG based IgG immune complexes assay revealed positivity in six out of eight neuritic, one out of nine TT, four out of ten BT, two out of ten BL, four out of ten LL leprosy cases, and two out of 24 healthy controls. IgM type of mycobacterial immune complexes were almost negligible. Capture of complexes using monoclonal antibody ML34 which is against lipoarabinomannan of M. leprae seems to work better than polyclonal anti-BCG antibody. The probable role of immune complexes in nerve damage needs to be evaluated, as very high levels of immune complexes are found in neuritic leprosy by both the assays. The above test would be useful in immunodiagnosis of neuritic leprosy and also in cases where antibody response is not detectable because of the formation of immune complexes.

Antigen-Antibody Complex↗

PubMed2000

IgA1 glycosylation and the pathogenesis of IgA nephropathy.

Explore the source record for details and available documents.

Antigen-Antibody Complex↗

PubMed2000

Influence of blood and synovial fluid immune complexes of patients with rheumatoid arthritis on production of nitric oxide and growth and viability of chondrocytes.

OBJECTIVE: To determine whether blood and synovial fluid (SF) immune complexes (IC) of patients with rheumatoid arthritis (RA) influence the production of nitric oxide (NO) and growth and viability of chondrocytes. METHODS: IC were precipitated and IgM and IgG were determined in the precipitates by ELISA and nephelometry, respectively. Primary cultures of bovine articular chondrocytes were incubated with the IC precipitates. After 48 h NO was determined as nitrite. After 7 days, growth was determined by incorporation of tritiated thymidine and viability was detected by neutral red uptake. RESULTS: Patient sera were positive in 8/12 for IgM IC and 9/12 for IgG IC, and 1/8 control sera was slightly positive for IgM IC. Seven of 12 SF samples were IgM IC and 10/12 IgG IC positive. With and without additional interleukin 1alpha (IL-1alpha) stimulation, NO production by chondrocytes was significantly higher with SF IC precipitates than with control serum precipitates (p = 0.03, p = 0.04, respectively). NO production by chondrocytes that were not stimulated with IL-1alpha was significantly increased with SF IC precipitates compared to RA serum IC precipitates (p = 0.03). SF IC significantly inhibited growth compared to control serum precipitates (p = 0.04) and RA serum IC (p = 0.012). Neutral red uptake by chondrocytes was significantly decreased when incubated with RA serum IC in comparison with control serum IC (p = 0.012) and SF IC (p = 0.006). With and without additional IL-1alpha stimulation, NO production by chondrocytes after incubation with SF derived IC was positively correlated to the Ritchie score (r = 0.8, r = 0.7, respectively) and the number of swollen joints (r = 0.8, r = 0.6, respectively). CONCLUSION: These results support the hypothesis that, especially in active RA, SF derived IC stimulate NO production and inhibit chondrocyte growth.

Antigen-Antibody Complex↗