Time window analysis and sorting.

J F Dunne

doi:10.1002/cyto.990120703

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Time window analysis and sorting.

J F Dunne

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Abstract

Flow cytometric hardware and procedures were developed to continuously analyse and to sort a particular time window in a kinetic response. The technique uses balanced air pressure to drive a stimulus-bead mixture from a vial to a t-junction where it mixes passively with cells. The t-junction is distanced from the flow cell and air pressure regulated so that the stimulation occurs at a fixed and adjustable time before the cells are interrogated by the laser beam. Practical applications of the device demonstrate utility with cells whose responses are seen in seconds or minutes. The device is easily implemented on any sorting flow cytometer.

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Animals Antibodies, Monoclonal Antigens, CD Bradykinin Calcium Cell Line, Transformed Equipment Design Flow Cytometry Flow Injection Analysis Hydrogen-Ion Concentration Kinetics Lasers Mice Rats Signal Transduction Journal Article

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J F Dunne. 1991. Time window analysis and sorting.. https://doi.org/10.1002/cyto.990120703

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