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PubMed · 16631357

Lactobacilli binding human A-antigen expressed in intestinal mucosa.

Hideaki Uchida·Hideki Kinoshita·Yasushi Kawai·Haruki Kitazawa·Koh Miura·Kenichi Shiiba·Akira Horii·Katsunori Kimura·Naoki Taketomo·Munehiro Oda·Takaji Yajima·Tadao Saito
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Abstract

Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.

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ABO Blood-Group SystemBacterial AdhesionBacterial ProteinsBiosensing TechniquesFecesHumansIntestinal MucosaLactobacillusProtein BindingSurface Plasmon ResonanceJournal ArticleResearch Support, Non-U.S. Gov't

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Hideaki Uchida, Hideki Kinoshita, Yasushi Kawai, Haruki Kitazawa, Koh Miura, Kenichi Shiiba, Akira Horii, Katsunori Kimura, Naoki Taketomo, Munehiro Oda, Takaji Yajima, Tadao Saito. 2006-03-30. Lactobacilli binding human A-antigen expressed in intestinal mucosa.. https://doi.org/10.1016/j.resmic.2006.03.001

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