Living cells as test tubes.

X Sunney Xie; Ji Yu; Wei Yuan Yang

doi:10.1126/science.1127566

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Living cells as test tubes.

X Sunney Xie·Ji Yu·Wei Yuan Yang

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Abstract

The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.

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Animals Cells Fatty Acids Gene Expression Lipid Metabolism Luminescent Proteins Molecular Motor Proteins Molecular Probe Techniques Proteins Quantum Dots Spectrum Analysis, Raman Journal Article Research Support, N.I.H., Extramural

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X Sunney Xie, Ji Yu, Wei Yuan Yang. 2006-04-14. Living cells as test tubes.. https://doi.org/10.1126/science.1127566

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