Gene expression analysis using filter cDNA microarrays.

Peter Simpson; Chris Jones; Alan Mackay; Sunil R Lakhani

doi:10.1385/1-59259-969-9:415

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Gene expression analysis using filter cDNA microarrays.

Peter Simpson·Chris Jones·Alan Mackay·Sunil R Lakhani

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Abstract

The analysis of gene expression patterns by filter-based complementary (c)DNA microarray remains an important technique in the molecular biology laboratory, despite the development of large-scale cDNA microarray analysis (see Chapter 27). This chapter provides an overview of the methods necessary to carry out the production of membrane-based cDNA arrays and the subsequent synthesis and hybridization of radiolabeled cDNA probes.

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Peter Simpson, Chris Jones, Alan Mackay, Sunil R Lakhani. 2006. Gene expression analysis using filter cDNA microarrays.. https://doi.org/10.1385/1-59259-969-9%3A415

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