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PubMed · 16441344

Oxylipin analysis methods.

Martin J Mueller·Laurent Mène-Saffrané·Christoph Grun·Kathrin Karg·Edward E Farmer
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Abstract

Practical guidelines for monitoring and measuring compounds such as jasmonates, ketols, ketodi(tri)enes and hydroxy-fatty acids as well as detecting the presence of novel oxylipins are presented. Additionally, a protocol for the penetrant analysis of non-enzymatic lipid oxidation is described. Each of the methods, which employ gas chromatography/mass spectrometry, can be applied without specialist knowledge or recourse to the latest analytical instrumentation. Additional information on oxylipin quantification and novel protocols for preparing oxygen isotope-labelled internal standards are provided. Four developing areas of research are identified: (i) profiling of the unbound cellular pools of oxylipins; (ii) profiling of esterified oxylipins and/or monitoring of their release from parent lipids; (iii) monitoring of non-enzymatic lipid oxidation; (iv) analysis of unstable and reactive oxylipins. The methods and protocols presented herein are designed to give technical insights into the first three areas and to provide a platform from which to enter the fourth area.

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EsterificationGas Chromatography-Mass SpectrometryLipidsOxidation-ReductionReference StandardsJournal ArticleResearch Support, Non-U.S. Gov't

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Martin J Mueller, Laurent Mène-Saffrané, Christoph Grun, Kathrin Karg, Edward E Farmer. 2006. Oxylipin analysis methods.. https://doi.org/10.1111/j.1365-313x.2005.02614.x

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