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PubMed · 16000077

Complex proteome prefractionation using microscale solution isoelectrofocusing.

Hsin-Yao Tang·David W Speicher
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Abstract

The proteomes of mammalian cells, tissues and biologic fluids are complex and consist of proteins present over a wide dynamic range. Current protein profiling technologies do not have the capacity to overcome the sample complexity for comprehensive analysis of complex proteomes. A common strategy to substantially expand protein profiling capacities is sample prefractionation. A prefractionation method developed in the authors' laboratory, microscale solution isoelectrofocusing, has resulted in a commercial product, the ZOOM IEF Fractionator, which provides a simple and convenient method for high-resolution separation of complex proteomes based upon their isoelectric points. Complex human samples such as cancer cells and biologic fluids can be fractionated into well-resolved fractions with minimal cross-contamination of proteins between adjacent fractions. This review focuses on the application of microscale solution isoelectrofocusing prefractionation and subsequent downstream strategies in expanding protein profiling capacities and mining low-abundance proteins of complex proteomes.

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AnimalsElectrophoresis, Gel, Two-DimensionalHumansIsoelectric FocusingMicrochemistryProteomeProteomicsSensitivity and SpecificityJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.Review

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Hsin-Yao Tang, David W Speicher. 2005. Complex proteome prefractionation using microscale solution isoelectrofocusing.. https://doi.org/10.1586/14789450.2.3.295

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