Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G Carter; Alexei A Sharov; Vincent VanBuren; Dawood B Dudekula; Condie E Carmack; Charlie Nelson; Minoru S H Ko

doi:10.1186/gb-2005-6-7-r61

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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G Carter·Alexei A Sharov·Vincent VanBuren·Dawood B Dudekula·Condie E Carmack·Charlie Nelson·Minoru S H Ko

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Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

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Animals Base Sequence Expressed Sequence Tags Gene Expression Profiling Genome Introns Mice Models, Genetic Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Open Reading Frames RNA, Messenger Restriction Mapping Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae Transcription, Genetic Journal Article

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Mark G Carter, Alexei A Sharov, Vincent VanBuren, Dawood B Dudekula, Condie E Carmack, Charlie Nelson, Minoru S H Ko. 2005-06-30. Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.. https://doi.org/10.1186/gb-2005-6-7-r61

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