Functional genomics using high-throughput RNA interference.

Dominique Vanhecke; Michal Janitz

doi:10.1016/s1359-6446(04)03352-5

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PubMed · 15708535

Functional genomics using high-throughput RNA interference.

Dominique Vanhecke·Michal Janitz

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Abstract

RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.

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Animals Gene Expression Profiling Gene Library Gene Silencing Genomics Humans Microarray Analysis RNA Interference RNA, Small Interfering Journal Article Research Support, Non-U.S. Gov't Review

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Dominique Vanhecke, Michal Janitz. 2005-02-01. Functional genomics using high-throughput RNA interference.. https://doi.org/10.1016/s1359-6446(04)03352-5

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