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PubMed · 15565682

Subsecond chiral separations on a microchip.

Natalia Piehl·Martin Ludwig·Detlev Belder
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Abstract

Fast chiral separation of DNS-amino acids could be realized using microchip electrophoresis with fluorescence detection. For this purpose, highly sulfated cyclodextrins (HS-gamma-CD) were used as chiral selectors enabling high selectivity. Even subsecond separation of DNS-tryptophan, DNS-norleucine, DNS-phenylalanine, DNS-methionine, and DNS-aspartic acid could be achieved. Baseline separation could be accomplished within 720 ms, which is the fastest separation of enantiomers reported to date. A more complex mixture consisting of three chiral DNS-amino acids could be separated within 3.3 s utilizing a separation length of only 7 mm and an electrical field strength of 2012 V/cm.

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Amino AcidsCyclodextrinsDansyl CompoundsElectrophoresis, MicrochipFluorescenceOptical RotationStereoisomerismJournal Article

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Natalia Piehl, Martin Ludwig, Detlev Belder. 2004. Subsecond chiral separations on a microchip.. https://doi.org/10.1002/elps.200406028

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