PubMed · 15481357
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J van Rooijen. 2004-09-15. [Darwin not against euthanasia].. https://pubmed.ncbi.nlm.nih.gov/15481357/
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Aerobic bacterial culture was used to compare the effectiveness of the current USDA footwear disinfection protocol for airplane passengers contacting livestock to a novel protocol. The current protocol consists of brushing and dipping shoe soles in 1% Virkon S. The number of bacteria was not different between shoes treated with the current protocol and untreated shoes. No shoes met the standard for disinfection after the current disinfection protocol was completed. The novel protocol consisted of brushing shoe soles, wiping soles with a cotton towel soaked in 1% Virkon S, and drying soles with paper towels. The number of bacteria was less (P<0.0001) on treated shoes compared to control shoes. Eighteen of 20 shoes (90%) cleaned using the novel protocol met the standard for disinfection. Direct comparison of the current and novel protocols found that the number of bacteria cultured was less (P<0.0001) after implementing the novel protocol compared to implementing the current protocol. Again, no shoes treated using the current protocol met the standard for disinfection after the current protocol was completed. Sixteen and 17 of 20 shoes (80--85%), respectively, met the standard for disinfection after the novel protocol was completed. Under conditions of this study, current US airport footwear disinfection protocols were inadequate to disinfect footwear when using aerobic bacteria as a marker for disinfection. We recommend implementation of the novel footwear disinfectant protocol for select passengers from international flights.
Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals were examined by polymerase chain reaction. LukS and lukF genes were detected in all 48 avian and 72 porcine isolates of S. aureus. LukE and lukD genes, located in a putative staphylococcal pathogenicity island (Sapln3/Saplm3), were recognized in 44 (91.7%) of 48 avian isolates, but these genes were not detected in porcine isolates. In 297 bovine isolates collected from mastitic cow's milk and bulk milk from dairy farms in two regions, lukM and lukF-PV(P83) genes in addition to lukS-lukF and lukE-lukD genes were detected in 100 (62.5%) of the 160 isolates from Ishikawa and in118 (86.1%) of the 137 isolates from Hokkaido. When the lysogeny of S. aureus bovine isolates was examined by treatment with mitomycin C, clearing of the culture due to cell lysis was observed in 34 (91.9%) of 37 lukM-lukF-PV(P83) genes--positive isolates. In addition, we isolated a novel lukM-lukF-PV(P83)-carrying (designated phiLukM), and revealed that the lukM-lukF-PV(P83) genes were located very close to an amidase gene on the temperate phage genomes. These results suggest horizontal transmission of lukM-lukF-PV(P83) genes by temperate bacteriophages in S. aureus of bovine origin.