PubMed · 15422955
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1950. COSMETICS.. https://pubmed.ncbi.nlm.nih.gov/15422955/
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A new method of analyzing kohl, a cosmetic eyeliner, using confocal Raman microscopy is reported. This technique offers an important alternative to conventional spectroscopic techniques that provide elemental/atomic composition. Raman spectra of three kohl samples have been measured between 150 and 3000 cm(-1) at room temperature. The main component of two kohl samples was found to be lead(II) sulfide (PbS). Kohl is used as a traditional cosmetic and remedy in the Middle East, Far East, and Northern Africa. Since kohl products contain very high concentrations of lead, they constitute a risk for public health, particularly for children.
In this work, a simple and reliable method for the simultaneous analysis of alpha-hydroxy acids such as tartaric, glycolic and lactic acids in cosmetic products was developed using capillary electrophoresis with indirect UV detection at 254 nm. A buffer solution containing 10 mmoll(-1) potassium phthalate (pH 4.1) and 0.5 mmoll(-1) cetyltrimethylammonium bromide as electroosmotic flow modifier allowed baseline resolution of the analytes in approximately 3 min. A few validation parameters of the proposed method include: good linearity for all compounds in the range from 10 to 100 mgl(-1) with coefficients of correlation larger than 0.9999. The average recoveries of tartaric, glycolic and lactic acids in commercial samples were 99.12, 99.41 and 99.43%, respectively. The method presented acceptable precision with average relative standard deviation of 0.54% (assay of commercial samples), 0.44% (peak area) and 0.16% (migration time).