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PubMed · 15163893

Interleukin-4 cellular gene therapy and osteoprotegerin decrease inflammation-associated bone resorption in collagen-induced arthritis.

Nathalie Saidenberg-Kermanac'h·Natacha Bessis·Delphine Lemeiter·Marie-Christine de Vernejoul·Marie-Christophe Boissier·Martine Cohen-Solal
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Abstract

To evaluate the respective action of IL-4, an anti-inflammatory cytokine, and OPG, an inhibitor of bone resorption, on the inflammatory process and the associated bone resorption in collagen-induced arthritis (CIA). After CIA induction, DBA/1 mice were treated with OPG or with IL-4 DBA/1 transfected fibroblasts or both OPG + IL-4. CIA significantly improved in IL-4 groups. OPG had no effect on arthritis clinical scores but histologic scores were reduced in OPG, IL-4, and OPG + IL-4 groups vs. nontreated CIA mice. OPG increased significantly BMD and decreased by 45% D-pyridinolin levels. Moreover association of IL-4 and OPG exerted an additive effect of BMD and resorption marker (-68%). Production of IFN-gamma in the supernatants of spleen cells was reduced in IL-4 treated mice. OPG had a moderate effect on IFN-gamma, but potentiated the inhibitory effect of IL-4. OPG and IL-4 prevent bone loss in CIA-mice model and could have additive effects on IFN-gamma secretion.

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AnimalsArthritisBone ResorptionCell LineCollagenDrug Therapy, CombinationFibroblastsGenetic TherapyGlycoproteinsInflammationInterferon-gammaInterleukin-4MaleMiceMice, Inbred DBAOsteoprotegerinReceptors, Cytoplasmic and NuclearReceptors, Tumor Necrosis FactorTransfectionJournal ArticleResearch Support, Non-U.S. Gov't

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Nathalie Saidenberg-Kermanac'h, Natacha Bessis, Delphine Lemeiter, Marie-Christine de Vernejoul, Marie-Christophe Boissier, Martine Cohen-Solal. 2004. Interleukin-4 cellular gene therapy and osteoprotegerin decrease inflammation-associated bone resorption in collagen-induced arthritis.. https://doi.org/10.1023/b%3Ajoci.0000029116.12371.bf

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