Search PubMed⌕ Search

PubMed · 15052357

Development of a multiplex microarray microsystem.

Abstract

A hybrid multiplex microarray microsystem has been developed that consists of 32 individually addressable array reaction chambers, supporting the use of multichannel pipettes for addition of up to 8 samples simultaneously. Discrimination between Campylobacter jejuni and Campylobacter coli bacteria was observed in DNA samples containing Campylobacter spp., with the same specificity and sensitivity as when compared to a full-size microarray. The spinloaded multiplex microarray microsystem described provides a novel and convenient test format for simultaneous low-density microarray analysis and is universally adaptable to other DNA, protein or small molecule microarray based applications.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Georgios Keramas, Gerardo Perozziello, Oliver Geschke, Claus B V Christensen. 2004-01-16. Development of a multiplex microarray microsystem.. https://doi.org/10.1039/b313472e

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Micellization of casein-graft-dextran copolymer prepared through Maillard reaction.

Casein is almost insoluble at around pH 4.6, which is its isoelectric point (pI). Grafting copolymer, casein-g-dextran, was prepared through the Amadori rearrangement of the Maillard reaction. The copolymer has a reversible pH sensitive property: micellization at the pI of casein forming a casein core and dextran shell structure and dissociation when pH differs from the pI. The micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. The micelles are very stable in aqueous solution and can be stored as lyophiled powder. The micelles are able to encapsulate hydrophobic compounds such as pyrene.

Biocompatible Materials↗

Synthesis and characterization of novel polyanhydrides with tailored erosion mechanisms.

We have designed a new synthesis route to create polyanhydrides based on monomers that contain hydrophilic entities within highly hydrophobic backbones. The method results in polyanhydrides that can be easily processed into drug-containing tablets. The synthesis, characterization, and erosion studies of polyanhydride copolymers based on 1,6-bis(p-carboxyphenoxy)hexane (CPH), which is highly hydrophobic, and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG), which has hydrophilic oligomeric ethylene glycol segments in the monomer unit, was performed using a combination of molecular spectroscopy, thermal analysis, gravimetry, and scanning electron microscopy. The studies demonstrate that by increasing the CPH content in the CPTEG:CPH copolymers, the erosion of the system can be tailored from bulk-eroding to surface-eroding mechanism. These systems have promise as protein carriers.

Biocompatible Materials↗

Influence of fluorescent and opalescent properties of resin composites on the masking effect.

The objective of this study was to determine the influence of fluorescent properties in the reflectance and transmittance modes and opalescent properties and translucency under ultraviolet (UV)-included and -excluded conditions on the masking effect of commercial resin composites quantitatively. Color and spectral distribution of seven resin composites (14 shades) of 1-mm thick were measured in the reflectance and transmittance modes under UV-included and -excluded conditions. For the fluorescence evaluation, subtraction spectra by the inclusion and exclusion of the UV component of the illumination in the reflectance and transmittance modes were calculated. Opalescence parameter (DeltaO*(ab)) was calculated as the difference in yellow-blue (Deltab*) and red-green (Deltaa*) coordinates between the reflected and transmitted colors under UV-included and -excluded conditions. Under UV-included and -excluded conditions, translucency parameter (TP) was calculated and masking effect was calculated as the color difference between a specimen over a black tile and black tile itself. Fluorescent and opalescent properties varied by the brand and shade of composites and measurement protocols. Masking effect was correlated with TP values when TP values of materials were obviously different. But when TP values were in the similar range, the opalescent property influenced the masking effect of resin composites. The influence of fluorescent property on masking effect was also confirmed, although the degree of correlation was very low.

Biocompatible Materials↗