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At least 19 recordsLinked to original sources

Comparison of the shear bond strength of a light-cured glass ionomer and a chemically cured glass ionomer for use as an orthodontic bonding agent.

Light-cured glass ionomers with an initial set of 20 seconds may produce higher initial bond strengths, as well as decreased sensitivity to moisture contamination and desiccation, than chemically cured glass ionomers making them attractive for use as orthodontic bonding agents. The purpose of this study was to determine and compare the shear bond strength of stainless steel orthodontic attachments to enamel with a light-cured glass ionomer (Zionomer) tested at 60 minutes and 24 hours, and a rapidly setting chemically cured glass ionomer (Ketac-Bond) tested at 60 minutes and 24 hours. Fifty-two recently extracted human premolars were randomly divided into four groups--1-hour and 24-hour light-cured glass ionomer groups and 1-hour and 24-hour chemically cured glass ionomer groups. Stainless steel lingual buttons were bonded to prepared enamel surfaces, and the samples were placed in a water bath at 37 degrees C until ready for testing. The shear bond strength of each sample was determined with a universal testing instrument. Results from the study conclude: (1) The mean shear bond strength of the light-cured glass ionomer is greater than that of the chemically cured glass ionomer at 1 and 24 hours. (2) The mean shear bond strength of both glass ionomers increases from 1 to 24 hours. (3) The mean shear bond strength of the light-cured glass ionomer is not significantly different from 1 to 24 hours, but the shear bond strength of the chemically cured glass ionomer cement is different.(ABSTRACT TRUNCATED AT 250 WORDS)

Acid Etching, Dental

Behaviour of fetal rat osteoblasts cultured in vitro on bioactive glass and nonreactive glasses.

We examined the behaviour of fetal rat osteoblasts cultured upon bioactive glass and nonreactive glasses, and the supposed stimulatory effects of bioactive glass on osteoblasts. Nonreactive glass cultures showed flattened cells with almost no dorsal ruffles. Bioactive glass cultures showed compact cells with dorsal ruffles and filapodia resulting in the formation of a denser cell layer. For confluent nonreactive glass cultures the osteoblast expression was mainly concentrated in the clustered cells which were formed upon the monolayer, whereas for confluent bioactive glass cultures the osteoblast expression was more generally distributed. The production of type I collagen, osteocalcin and an osteoblast-specific antigen was shown by immunocytochemistry for all cultures, although differences in distribution were observed. The bioactive layer of bioactive glass is responsible for a better osteoblast-like morphology, a higher proliferation rate and generally a better osteoblast expression.

Alkaline Phosphatase

Role of the glass phase in bioactive glass-ceramics.

Glass-ceramics, or composites with a glass-ceramic matrix prepared by controlled crystallization, almost invariably contain a residual glass phase. A suitable composition for the residual glass phase of bioactive glass-ceramics can be found approximately and controlled on the basis of calculation of a structural parameter Y, which in the simplified concept of the glass structure corresponds to the mean number of bridging oxygens per polyhedron in the glass lattice.

Biocompatible Materials

A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide.

Glass wool, hydrophilic cotton wool, non-electrically charged BIO-GEL P2 and common tissue paper columns were used to purify trypomastigotes from a mixed Trypanosoma cruzi population grown in axenic culture medium. With all these columns, highly purified (up to 98%) trypomastigote preparations were obtained. Trypomastigote yields from cotton wool, BIO-GEL P2 and common tissue paper columns were not as high as from glass wool columns, from which yields varied from 69 to 80%. Purification on glass wool did not affect trypomastigote infectivity or virulence. Dead trypomastigotes could not be purified on glass wool columns. A glass-adherent amphiphilic peptide of 45 kDa, present in the cell membrane, was isolated from epimastigote but not from trypomastigote preparations.

Animals

A study of fracture and inelastic behavior of bioactive glass-ceramics and glasses.

The fracture and inelastic behavior of A-W glass-ceramics, phosphate glasses, silicate glasses, and borate glasses were determined in simulated body fluid (SBF), kerosene, and water. By using the stable crack growth technique, an inelastic behavior was observed on the diagram of load versus load-point displacement. From these studies, it was suggested that the inelastic behavior of bioactive glass-ceramics was produced by the plastic deformation of glassy phase on the grain boundary.

Apatites

Glass fibre versus non-glass fibre splinting bandages.

We have assessed the current range of synthetic splinting bandages, comparing glass with non-glass fabrics and plaster-of-Paris. Physical and mechanical tests have been carried out and the opinions of patients, volunteers and orthopaedic staff were recorded. Modern bandages have some better properties than standard plaster bandage but do not conform as well, are more expensive and potentially more hazardous. However, non-glass bandages are lighter, less brittle, more radiolucent and less hazardous than glass fibre bandages and are preferred by both patients and applicators.

Attitude to Health

The biocompatibility of glass-poly(alkenoate) (Glass-Ionomer) cements: a review.

The literature describing the biocompatibility of glass-poly(alkenoate) ('Glass-Ionomer') cements has been reviewed. This literature shows that these materials have generally good biocompatibility for both dental and orthopaedic use, this latter observation being very recent. There have, though, been a few reports showing that in certain circumstances these materials may cause pulpal irritation and the reasons for these particular findings are considered. Following discussion of the biocompatibility of Glass-Ionomer cements, consideration is given to the likely underlying causes of this feature. Three factors are identified as contributing to the biocompatibility of these cements. They are: (i) minimal exotherm on setting; (ii) rapid neutralization following mixing; and (iii) slow release of ions which are generally biologically beneficial or, at least, benign. This last point is considered in some detail. Previous studies of leaching of ions from Glass-Ionomer cements have shown that only inorganic species are released. The biological effects of each of these inorganic ions are described and their influence on biocompatibility discussed.

Aluminum

Microleakage of glass ionomer/composite resin restorations: a laboratory study. 1. The influence of glass ionomer cement.

Acid-etching of enamel margins, often combined with bevelling and use of a low viscosity resin, has resulted in improved sealing of composite restorations. When enamel is absent and margins involve dentine or cementum, microleakage is more often observed. The use of glass ionomer cement, as both a restorative material and as a base combined with a veneer of composite resin, has been advocated as a means of minimizing microleakage where margins are placed in dentine or cementum. Four restorative techniques incorporating glass ionomer cement were used, in vitro, to restore cervical cavities. After thermal cycling, dye penetration was scored along occlusal and gingival margins. In all instances the gingival margin exhibited a less reliable seal than the occlusal margin. The sandwich restorations produced a superior seal at both occlusal and gingival margins when compared with glass ionomer restorative cement.

Acid Etching, Dental

Modifications of glass microelectrodes: a self-filling and a semifloating glass microelectrode.

A simple and inexpensive method for constructing a double capillary was developed for preparing a self-filling glass microelectrode. The modified method uses a thin capillary instead of a bundle of glass fibers and fuses it to the outer capillary wall with flame before pulling the latter with a puller. The validity of the new electrode was proven by measuring the proximal cell PD of the rat kidney slices (mean of the stable cell PDs lasting longer than 30 min,--69 mV). A semifloating electrode was modified from the original floating electrode to be applicable to the in vivo kidney preparation whose pulsatile and respiratory movements had hindered yielding a stable cell PD. This electrode is constructed simply by replacing the shaft of the Ling-Gerard glass microelectrode with a length of flexible silcone-rubber tubing. The cell PD recorded by this electrode is found to be more stable and can withstand minor tissue distortion in mammalian kidney cells in vivo and also in such a contractile tissue as the secretory coil of the eccrine sweat gland.

Animals

Platelet retention in glass bead columns: adhesion to glass and subsequent platelet-platelet interactions.

In normal heparinized blood, the retention of platelets in glass bead columns was low in the first 1 or 2 ml, increasing to more than 80% by the 4th or 5th ml. Prior flushing of the columns with platelet-poor plasma or saline lowered retention in all 5 ml. Additional studies were carried out with a two-stage procedure in which a sample of blood (A) was pumped through a column, immediately flushed out with saline or plasma, and followed by a second blood sample (B). When as little as 1 ml of blood A preceded the flushing solution, retention was very high in all 5 ml of the subsequent blood B. This enhancement of retention in B occurred, providing blood A contained platelets (other than thrombasthenic), fibrinogen, and adequate divalent cations. Enhancement did not require von Willebrand factor (vWF) in A, nor was ADP necessary, since enhancement occurred even when heparinized blood as A contained prostaglendin E1 (PGE1) or creatine phosphokinase with creatine phosphate (CPK-CP). However, the presence of PGE1 or CPK-CP in the plasma used to flush the columns prevented the enhancement of retention in the first milliliter of B. Retention in the first milliliter of B (following normal blood as A and saline or normal plasma for flushing) was high when B was afibrinogenemic, moderately high when B contained PGE1 or CPK-CP, and low in thrombasthenic, EDTA, or vWF-deficient blood. Retention declined in subsequent milliliters of PGE1 or CPK-CP blood and remained low in thrombasthenic, vWF-deficient, or EDTA blood. Our findings suggest that (1) the platelets in A adhere to glass; this adhesion requires fibrinogen but not vWF or ADP; (2) the adherent platelets release ADP and become sticky; (3) adhesion of platelets in the first milliliter of B to the sticky platelets from A requires vWF and divalent cations but not ADP; (4) retention is maintained thereafter by repetitive platelet-platelet interactions involving ADP release, alteration of adherent platelets by released ADP, and adhesion of further platelets to these ADP-altered platelets which requires vWF.

Adenosine Diphosphate

The clinical performance of a glass polyalkenoate (glass ionomer) cement used in a 'sandwich' technique with a composite resin to restore Class II cavities.

The use of glass polyalkenoate (GPA) cement in conjunction with composite resin using an acid etch technique is now well known and a 'sandwich' of tooth/GPA cement/composite resin has been recommended to form the cervical seal at the base of approximal boxes in Class II cavities. This paper reports on the 2-year results of a controlled clinical trial using a commercial GPA lining cement. The trial was designed to evaluate the efficacy of this 'sandwich' technique. Sixty-four restorations in the mouths of 22 patients were evaluated during the 2.5 year period of the trial. The composite resin component of the restorations performed well. Five restorations failed, all in the region of the approximal box. Four failures were related to the exposed GPA cement component of the 'sandwich'. Failure was probably related to placement difficulties of the technique. The use of GPA cement laminated with composite resin when the GPA cement was enclosed within the final restoration appeared to be a successful technique.

Adult

Anti-IgG immobilized controlled-pore glass. Thionyl chloride-activated succinamidopropyl-glass as a covalent immobilization matrix.

Rabbit anti-bovine IgG was covalently immobilized on thionyl chloride-activated succinamidopropyl controlled-pore glass (CPG) beads (3000 A pore diam; 120/200 mesh). Thionyl chloride-activated beads remained stable for over 1 y retaining full capability of immobilizing protein upon recirculation of protein solution. The immobilized anti-bovine IgG is capable of binding IgG with a dissociation constant (Kd) of 9.45 x 10(-7) M and a capacity of 0.85 g/L of matrix. A column of immobilized anti-IgG is able to remove all detectable contaminating IgG from partially purified enzyme preparations of sulfhydryl oxidase and gamma-glutamyltransferase as determined by ELISA and Western blot. The column matrix could be regenerated by washing with 0.1M acetic acid, pH 2.8.

Animals