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PubMed · 15007428

Sequence-selective DNA detection using multiple laminar streams: a novel microfluidic analysis method.

Kenichi Yamashita·Yoshiko Yamaguchi·Masaya Miyazaki·Hiroyuki Nakamura·Hazime Shimizu·Hideaki Maeda
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Abstract

On-site detection methods for DNA have been demanded in the pathophysiology field. Such analysis requires a simple and accurate method, rather than high-throughput. This report describes a novel microfluidic analysis method and its application for simple sequence-selective DNA detection. The method uses a microchannel device with a serpentine structure. Sequence-specific binding of probe DNA can be detected at one side of the microchannel. This method is capable of sequence-specific detection of DNA with high accuracy. Single base mutations can also be analyzed. Combination of laminar stream and laminar secondary flow in the microchannel enable specific detection of probe-bound DNA.

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Base SequenceBinding SitesDNA PrimersDNA ProbesFluorometryGenetic TechniquesMicrofluidicsSequence Analysis, DNAJournal ArticleResearch Support, Non-U.S. Gov't

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Kenichi Yamashita, Yoshiko Yamaguchi, Masaya Miyazaki, Hiroyuki Nakamura, Hazime Shimizu, Hideaki Maeda. 2003-12-02. Sequence-selective DNA detection using multiple laminar streams: a novel microfluidic analysis method.. https://doi.org/10.1039/b311571b

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