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Biomedical subjects

Hazime Shimizu

Publications and source records attributed to Hazime Shimizu.

6 recordsLinked to original sources
PubMed2004

Direct observation of long-strand DNA conformational changing in microchannel flow and microfluidic hybridization assay.

Conformational control of macromolecules is useful for efficient chemical and biochemical reactions. This article reports a direct observation method for macromolecules, such as long-strand DNA, in microchannel flow as well as a simple method for stretching DNA strands by microfluidics. Stretching and orientation of DNA molecules by control of flow within a microchannel was observed by optical microscopy. This DNA stretching is explained by coil-stretch transition of polymer molecules. This technique is useful for creating chemical reactions with macromolecules. It offers high selectivity and efficiency that are impossible to achieve in bulk solution. We also demonstrate that our microfluidic stretching method can accomplish efficient hybridization of long-strand DNA. This method will be useful for direct hybridization assay of long-strand DNA.

DNA↗
PubMed2004

Application of a microfluidic reaction system for CdSe nanocrystal preparation: their growth kinetics and photoluminescence analysis.

We used a microreactor for CdSe nanocrystal preparation and explored the effects of experimental conditions on the properties of the products. The particle growth kinetics and photoluminescence properties of the nanocrystals showed identical trends to previous reports, indicating the efficiency of the current method for analysis of rapid nanocrystal synthesis as well as industrial production of CdSe nanocrystals.

Journal Article↗
PubMed2003

Continuous synthesis of CdSe-ZnS composite nanoparticles in a microfluidic reactor.

ZnS-coated CdSe composite particles have been continuously synthesized in a microfluidic reactor. By using this system, CdSe particles and a ZnS coating can be produced in sequence, and the particle size and layer thickness can be directly adjusted by the residence time. It demonstrated that the continuous synthesis in the microreactor was a simple and efficient way to prepare composite particles with different structures and determine the optimized experimental conditions.

Journal Article↗
PubMed2003

Sequence-selective DNA detection using multiple laminar streams: a novel microfluidic analysis method.

On-site detection methods for DNA have been demanded in the pathophysiology field. Such analysis requires a simple and accurate method, rather than high-throughput. This report describes a novel microfluidic analysis method and its application for simple sequence-selective DNA detection. The method uses a microchannel device with a serpentine structure. Sequence-specific binding of probe DNA can be detected at one side of the microchannel. This method is capable of sequence-specific detection of DNA with high accuracy. Single base mutations can also be analyzed. Combination of laminar stream and laminar secondary flow in the microchannel enable specific detection of probe-bound DNA.

Base Sequence↗
PubMed2003

Synthesis of chemotactic peptide analogs with a photoaffinity cross-linker as new probes for analysis of receptor-ligand interaction.

Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with p-benzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.

Biotin↗