Reprogramming in nuclear transfer.

Alice Jouneau; Jean-Paul Renard

doi:10.1016/j.gde.2003.08.007

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Reprogramming in nuclear transfer.

Alice Jouneau·Jean-Paul Renard

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Abstract

Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids. These studies suggest that the pluripotent character of one nucleus is dominant over that of the other nucleus. The development of nuclear transfer embryos shows that there are several restriction points and that the extra-embryonic lineages may be the primary source of death. Successful reprogramming depends on the balance between epigenetic modifications and the regulative properties of development.

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Animals Cell Nucleus Cloning, Organism Cytoplasm Epigenesis, Genetic Mice Nuclear Transfer Techniques Journal Article Research Support, Non-U.S. Gov't Review

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Alice Jouneau, Jean-Paul Renard. 2003. Reprogramming in nuclear transfer.. https://doi.org/10.1016/j.gde.2003.08.007

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