PubMed · 14166232
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H G BREDEMYER. 1964. AXES AND ANGLES.. https://pubmed.ncbi.nlm.nih.gov/14166232/
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With few exceptions, the shells (capsids) of sphere-like viruses have the symmetry of an icosahedron and are composed of coat proteins (subunits) assembled in special motifs, the T-number structures. Although the synthesis of artificial protein cages is a rapidly developing area of materials science, the design criteria for self-assembled shells that can reproduce the remarkable properties of viral capsids are only beginning to be understood. We present here a minimal model for equilibrium capsid structure, introducing an explicit interaction between protein multimers (capsomers). Using Monte Carlo simulation we show that the model reproduces the main structures of viruses in vivo (T-number icosahedra) and important nonicosahedral structures (with octahedral and cubic symmetry) observed in vitro. Our model can also predict capsid strength and shed light on genome release mechanisms.
We demonstrated a microfluidic device for rapidly generating complex mixtures of 32 stock reagents in a 5-nl reactor. This "formulation chip" is fully automated and allows thousands of experiments to be performed in a single day with minimal reagent consumption. It was applied to systematically study the phase behavior of the protein xylanase over a large and complex chemical space. For each chemical formulation that demonstrated a pronounced effect on solubility, the protein phase behavior was completely mapped in the chip, generating a set of empirical phase diagrams. This ab initio phase information was used to devise a rational crystallization screen that resulted in 72-fold improvement in successful crystallization hits compared with conventional sparse matrix screens. This formulations tool allows a physics-based approach to protein crystallization that may prove useful in structural genomics efforts.
Hounsell and Wilkinson (1997 Phys. Med. Biol. 42 1737-49) have demonstrated that the concept of a scatter-plane source can accurately describe head scatter in irregularly shaped fields produced by the MLC of an Elekta linear accelerator. In these linacs, the solid jaw is below the MLC set and plays no part on defining the view of the main source of head scatter, the flattening filter. A more complicated situation arises for linear accelerator head designs which incorporate a solid upper jaw pair with the MLC jaws further from the source. Application of the Hounsell and Wilkinson technique to these accelerator designs does not achieve the accuracy in the Elekta application. We present a generalized extension to Hounsell and Wilkinson's model and present results for typical treatment field shapes on a Siemens Primus linear accelerator.