PubMed · 1392799
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P D Marsden. 1992-05-02. Cholera.. https://doi.org/10.1136/bmj.304.6835.1170
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Two novel aerobic bacterial strains, designated SGZ-38T and sgz301269T, were isolated from the root of Pennisetum sp. and paddy soil, respectively. Strain SGZ-38T grew at 10-40 ℃ (optimum 30 °C) and pH 5.0-12.0 (optimum 6.5) and tolerated up to 1.0% NaCl (w/v), whereas strain sgz301269T grew at 15-37 °C (optimum 30 °C), pH 5.0-9.5 (optimum 7.0) and 0-2% NaCl (optimum 0%). Phylogenetic trees based on the 16S rRNA gene and genomes placed both strains into distinct lineages, forming separated clades from their closest relatives. Strain SGZ-381T exhibited the highest 16S rRNA gene similarities to "Neorhizobium deserti" ACCC 61627T (97.4%), and strain sgz301269T had the highest 16S rRNA gene sequence similarity to Neotabrizicola shimadae N10T (97.6%). The respiratory quinone in both strains was ubiquinone-10. The main fatty acids of SGZ-381T were Summed feature 8, Summed feature 2 and C16:0, whereas strain sgz301269T included C10:0 3OH, C18:0 3OH and Summed feature 8. The DNA G+C content of SGZ-381T and sgz301269T was 62.1% and 65.5%, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between each strain and their respective closest species were 74.6% and 20.1%, 75.3% and 17.4% respectively, below the thresholds for species delineation. Based on the comprehensive chemotaxonomic, phylogenetic, and phenotypic evidence, proposed names of the novel strains are Neorhizobium fuzhouense sp. nov. (type strain SGZ-381T=GDMCC1.4207T=JCM 36770T), Neotabrizicola paludis sp. nov. (type strain sgz301269T=MCCC 1K09178T=KCTC 8856T).
The Communicable Disease Control Branch of the South Australian Health Commission received 45 laboratory notifications of Salmonella between 23 December 1996 and 17 January 1997. A rapid screening test, undertaken by the Institute of Medical and Veterinary Sciences, Adelaide, was the first indication that this was more than one outbreak, prompting the establishment of separate investigations. Three Salmonella Typhimurium (S. Typhimurium) phage types were subsequently identified. Investigations are continuing into an outbreak of S. Typhimurium phage type (PT) 64, while investigations failed to identify any association between four cases of PT 44. As of 12 February 1997, 71 notifications had been confirmed as S. Typhimurium PT 135. Epidemiological investigations found this outbreak was associated with consumption of bread rolls with a meat filling distributed through local Asian grocery stores from a home-based manufacturer. The product was voluntarily withdrawn and there have been no new cases of PT 135.
We have developed a semiquantitative homogeneous fluorescence assay that combines polymerase chain reaction (PCR) amplification with direct fluorescence detection (HF-PCR). The assay eliminates the need to perform gel electrophoresis on test samples. Using a set of Salmonella-specific primers, this system was used to verify suspect colonies from culture plates as Salmonella. The fluorescence signal is generated by a nucleic acid dye, YO-PRO-1, that is included in the amplification reaction. This homogeneous PCR assay was used to test 84 Salmonella strains picked from selective culture plates. All data indicated positive results when compared with 17 non-Salmonella strains (in general, Citrobacter, Hafnia, Proteus, and Escherichia). The HF-PCR assay is a sensitive, simple, accurate, and reproducible method that correlates well with size-exclusion high-performance liquid chromatography and gel electrophoresis techniques as a means to monitor PCR-mediated DNA amplification. This assay can confirm suspect colonies within 2.5 h.