tRNA recognition by CCA-adding enzyme.

Yukihide Tomari; Tsutomu Suzuki; Takuya Ueda

doi:10.1093/nass/2.1.77

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tRNA recognition by CCA-adding enzyme.

Yukihide Tomari·Tsutomu Suzuki·Takuya Ueda

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Abstract

The CCA-adding enzyme [ATP (CTP): tRNA nucleotidyltransferase (EC. 2.7.7.25)] catalyzes the addition of the conserved CCA sequence to the 3'-terminus of tRNAs. It is known that CCA-adding enzymes from all three kingdoms recognize the elbow region of L-shape tRNA formed by the tertiary interaction between T-loop and D-loop. We here demonstrate that C. elegans mitochondrial CCA-adding enzyme has broad substrate specificity toward nematode mitochondrial tRNAs which lack entire T- or D-arms, by cloning and characterizing the recombinant protein.

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Animals Base Sequence Caenorhabditis elegans Nucleic Acid Conformation RNA Nucleotidyltransferases RNA, Transfer Substrate Specificity Journal Article

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Yukihide Tomari, Tsutomu Suzuki, Takuya Ueda. 2002. tRNA recognition by CCA-adding enzyme.. https://doi.org/10.1093/nass%2F2.1.77

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