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PubMed · 12452569

Label-free bioaffinity detection using terahertz technology.

Samuel P Mickan·Abdellah Menikh·Haibo Liu·Carmen A Mannella·Robert MacColl·Derek Abbott·Jesper Munch·X C Zhang
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Abstract

We report the first use of differential terahertz time-domain spectroscopy for bioaffinity sensing. Binding is observed by measuring the transmission of a thin layer of biotin bound to the sensor protein avidin. We demonstrate the THz wave transmission of a sub-micron-thick film and sensitivity to 0.1 microg cm(-2) of biotin. These results point the way for a host of biosensor applications using T-rays, or pulsed far-infrared (FIR) radiation.

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Affinity LabelsAvidinBiosensing TechniquesBiotinElectromagnetic PhenomenaEquipment DesignMacromolecular SubstancesMembranes, ArtificialMicrowavesProtein BindingRefractometrySensitivity and SpecificitySpectrum AnalysisSurface PropertiesEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Samuel P Mickan, Abdellah Menikh, Haibo Liu, Carmen A Mannella, Robert MacColl, Derek Abbott, Jesper Munch, X C Zhang. 2002-11-07. Label-free bioaffinity detection using terahertz technology.. https://doi.org/10.1088/0031-9155%2F47%2F21%2F317

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