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PubMed · 12181645

A microarray-based method for detecting methylated loci.

Izuho Hatada·Azusa Kato·Sumiyo Morita·Yayoi Obata·Kayuri Nagaoka·Akira Sakurada·Masami Sato·Akira Horii·Atsumi Tsujimoto·Kenichi Matsubara
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Abstract

CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray.

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AnimalsCpG IslandsDNA MethylationHumansMiceOligonucleotide Array Sequence AnalysisStem CellsTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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Izuho Hatada, Azusa Kato, Sumiyo Morita, Yayoi Obata, Kayuri Nagaoka, Akira Sakurada, Masami Sato, Akira Horii, Atsumi Tsujimoto, Kenichi Matsubara. 2002. A microarray-based method for detecting methylated loci.. https://doi.org/10.1007/s100380200063

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