PubMed · 11569219
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D K Mccarthy, R H Gregg. 2001. Making waves.. https://pubmed.ncbi.nlm.nih.gov/11569219/
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Knowledge of the transport pathways of metals into aquatic organisms is paramount in determining the metal's potential mechanism of toxicity. To determine the relative importance of water and food as cadmium (Cd) sources for the cladoceran Daphnia magna grazing on the algae Chlorella vulgaris, we measured cadmium accumulation and toxicity (feeding inhibition and survival) in three genetically different clones of D. magna subsequent to water, food, and water and food exposures. We found that Cd uptake from water and food was independent of source and additive in effect, with D. magna juveniles accumulating twice as much Cd from water than from food (algae). However, the efficiency with which Cd was assimilated by D. magna from its algal diet was much higher (10%) than from water (0.3%). Uptake and toxic responses were inversely related: tolerant clones accumulated more Cd. As a consequence, models based on uptake of metals from the combined routes of water and food may be reliable to predict metal dynamics in the field, but may fail to predict toxic effects since tolerance to metals is not necessarily linked to reduced total uptake of metals.
Nodularin (NODLN) is a cyclic pentapeptide hepatotoxin that is regularly produced in high amounts by the cyanobacterium Nodularia spumigena in the Baltic Sea, and can bioaccumulate in Baltic biota. Baltic sea trout (Salmo trutta m. trutta L.) were exposed orally to a single dose of food containing NODLN (125 mg/kg ww) from N. spumigena (strain AV1, from the Baltic Sea). The level of exposure was 210-620 (average 440) microg NODLN per kg bw. Based on an 8-day survey under laboratory conditions, NODLN-like compounds accumulated in trout liver, with increasing liver concentrations (from 19 microg/kg on day 1 up to 1200 microg/kg on day 8 as measured with the EnviroLogix ELISA kit) during the experiment. Thus, accumulation of NODLN-like compounds in liver increased from 0.05% of the total NODLN dose administered on day 1 to 0.53% on day 8. However, the ELISA test kit is also sensitive to metabolites of algal hepatotoxins. In the HPLC chromatograms, no NODLN peak was detected after 24 h that also suggested NODLN absorbed in trout was metabolized or bound rapidly. According to ELISA, NODLN-like compounds also accumulated in trout muscle in lower quantities (from 125 to 34 microg/kg dw). Histopathology revealed complete loss of liver architecture after 1-2 days of the single oral dose. From day 4 to 8, there was partial recovery of liver cells. NODLN did not affect thiamine levels or water content of trout liver. The results showed that NODLN rapidly induces severe but reversible liver damage. Apparently NODLN accumulated in trout liver from cyanobacteria in the intestine, but was detoxified rapidly. On the basis of discrepancies between the histopathology and ELISA, and on the other hand, between the HPLC and ELISA methods, analysis of NODLN and its metabolites in biological tissue needs to be improved.
The addition of poly(ethylene glycol) and salts to clarified cell lysates of Thiosphaera pantotropha increases sorption of microbial proteins into dextran hydrogels, consistent with the thermodynamics of aqueous two-phase extraction. Addition of 12 wt% PEG-10,000 to the lysate increased total sorption of protein by the dextran gel from 5.2 mg/g dextran to 37 mg/g; addition of either 0.1 M potassium iodide or tetrabutylammonium fluoride along with PEG to the lysate increased protein sorption to more than 63 mg/g, a 12-fold increase. SDS-PAGE demonstrated that the type of salt added controls which proteins are absorbed by the gel. Previously demonstrated only with model solutions, these results suggest another approach to recovery and separation strategies for proteins produced by fermentation.