Ras-dependent cell cycle commitment during G2 phase.

M Hitomi; D W Stacey

doi:10.1016/s0014-5793(01)02115-9

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Ras-dependent cell cycle commitment during G2 phase.

M Hitomi·D W Stacey

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Abstract

Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next cell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase.

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Animals Cell Cycle Cyclin D1 G2 Phase Models, Biological Resting Phase, Cell Cycle S Phase ras Proteins Journal Article Research Support, U.S. Gov't, P.H.S.Review

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M Hitomi, D W Stacey. 2001-02-16. Ras-dependent cell cycle commitment during G2 phase.. https://doi.org/10.1016/s0014-5793(01)02115-9

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