Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.

M Ohsawa; S L Dun; L F Tseng; J Chang; N J Dun

doi:10.1016/s0014-2999(00)00374-5

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Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.

M Ohsawa·S L Dun·L F Tseng·J Chang·N J Dun

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Abstract

Immunohistochemical studies with the use of an antiserum against the cocaine- and amphetamine-regulated transcript (CART) peptide-(55-102) showed an abundance of CART-immunoreactive fibers in the mouse dorsal horn laminae I and II. A few CART-positive somata were scattered in the dorsal horn and around the central canal. Intrathecal injection of the CART peptide-(55-102) at doses 3, 10 and 100 ng caused a dose-dependent and significant decrease of paw withdrawal latency; whereas, saline injection was without significant effect. Our results provide the first evidence that CART-immunoreactive fibers are present in the dorsal horn and that the peptide administered intrathecally produces hyperalgesia, as assessed by paw withdrawal latency in mice.

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Animals Dose-Response Relationship, Drug Hindlimb Immunohistochemistry Injections, Spinal Male Mice Mice, Inbred ICR Nerve Tissue Proteins Pain Pain Measurement Peptide Fragments Reaction Time Spinal Cord Cocaine- and Amphetamine-Regulated Transcript Protein Journal Article Research Support, U.S. Gov't, P.H.S.

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M Ohsawa, S L Dun, L F Tseng, J Chang, N J Dun. 2000-07-07. Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.. https://doi.org/10.1016/s0014-2999(00)00374-5

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